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KMID : 0604520130390020159
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Journal of the Society of Cosmetic Scientists of Korea
2013 Volume.39 No. 2 p.159 ~ p.166
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The Inhibitory Effects of Alnus Japonica Steud. Extract on Melanogenesis
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Lee Jun-Young
Lim Kyung-Ran
Jung Taek-Kyu
Yoon Kyung-Sup
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Abstract
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In order to develop new skin whitening agents, we prepared the EtOAc layer (AJE) after enzyme treatment of 75% EtOH extract of the Alnus Japonica Steud. We measured their tyrosinase inhibitory activity in vitro and melanin synthesis inhibitory activity in B16-F1 melanoma cells. They did not show inhibitory activity against mushroom tyrosinase but showed melanin synthesis inhibitory activity in a dose-dependent manner. In a melanin synthesis inhibition assay, AJE suppressed melanin production up to 52% at a concentration of 40 μg/mL. To elucidate the mechanism of the inhibitory effects of AJE on melanogenesis, we measured expression of melanogenesis-related proteins by the western blot assay. As a result, AJE suppressed the expression of tyrosinase related protein 1 (TRP-1) and microphthalmia associated transcription factor (MITF). Moreover, AJE increased the expression of phosphorylated extracellular signal- regulated kinase (p-ERK). These results conclude that ERK activation by AJE reduces melanin synthesis via MITF downregulation and is subsequent to the inhibition of TRP-1 expression. Therefore, we suggest that AJE could be used as active ingredients for skin whitening.
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KEYWORD
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Alnus Japonica Steud, melanogenesis, TRP-1, MITF, ERK activation
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